BIOFLOC TECHNOLOGY: A SUITABLE TOOL FOR BLUE REVOLUTION IN INDIA
Biofloc technology (BFT) is considered the new “blue revolution” in aquaculture. The concept of rapid increase in the production of fish and marine product through package programme is called as blue revolution. It was launched in India during the seventh Five-year plan (1985-1990) when the Central Government sponsored the Fish Farmers Development Agency (FFDA). It has brought improvement in aquaculture by adopting new techniques of BFT,fish breeding, fish rearing, fish marketing, and fish export.
Blue Revolution, the Neel Kranti Mission has the vision to achieve economic prosperity of the country and the fishers and fish farmers as well as contribute towards food and nutritional security through full potential utilization of water resources for fisheries development in a sustainable manner, keeping in view the bio-security and environmental concerns. The Neel Kranti Mission, 2016 (NKM 16), being the year in which the Vision has been given by the Prime Minister will have multi-dimensional approach to all activities concerned with development of the fisheries sector as modern world class industry in India. It will focus on tapping the full production potential and enhance productivity substantially from aquaculture and fisheries resources, both inland and marine. Substantially increasing the share of Indian fisheries in the export area would be a key goal. It will ensure doubling the income of the fishers and fish farmers with inclusive participation of the socio-economically weaker sections and ensure sustainability with environment and biosecurity. The Biofloc technology (BFT) can play a pivotal role in achieving the goal of blue revolution in India.
The Biofloc technology enhances the production and productivity in fish culture, by its contribution to the supply of good quality fish juveniles, the latter being one of the most important inputs in the production systems. Biofloc technology could support the supply of good quality seeds by improving the reproductive performance of aquaculture animals and by enhancing the larval immunity and robustness .The application of biofloc technology in grow out systems of some aquaculture species could improve net productivity by 8–43%, relative to the non‐biofloc control (traditional with water exchange, clear water system or recirculating aquaculture system). Biofloc technology (BFT) has been successfully implemented in aquaculture especially shrimp farming owing to economical, environmental, and marketing advantages over the conventional culture system. Compared to conventional aquaculture techniques, biofloc technology provides a more economical alternative and sustainable technique in terms of minimal water exchange and reduced feed input making it a low-cost sustainable technology for sustainable future aquaculture development .
Biofloc systems were developed to improve environmental control over production. In places where water is scarce or land is expensive, more intensive forms of aquaculture must be practiced for cost-effective production. There are strong economic incentives for an aquaculture business to be more efficient with production inputs, especially the most costly (feed) and most limiting (water or land). High-density rearing of fish typically requires some waste treatment infrastructure. At its core, biofloc is a waste treatment system. Biofloc systems were also developed to prevent the introduction of disease to a farm from incoming water. In the past, standard operation of shrimp ponds included water exchange (typically 10 percent per day) as a method to control water quality. In estuarine areas with many shrimp farms practicing water exchange, disease would spread among farms. Reducing water exchange is an obvious strategy for improving farm biosecurity. Shrimp farming began moving toward more closed and intensive production where waste treatment is more internalized. Biofloc systems use a counter-intuitive approach— allow or encourage solids and the associated microbial community to accumulate in water. As long as there is sufficient mixing and aeration to maintain an active floc in suspension, water quality can be controlled. Managing biofloc systems is not as straightforward as that, however, and some degree of technical sophistication is required for the system to be fully functional and most productive.
Composition and nutritional value of bioflocs
Bioflocs are aggregates (flocs) of algae, bacteria, protozoans, and other kinds of particulate organic matter such as feces and uneaten feed. Each floc is held together in a loose matrix of mucus that is secreted by bacteria, bound by filamentous microorganisms, or held by electrostatic attraction (Fig. 1). The biofloc community also includes animals that are grazers of flocs, such as some zooplankton and nematodes. Large bioflocs can be seen with the naked eye, but most are microscopic. Flocs in a typical greenwater biofloc system are rather large, around 50 to 200 microns, and will settle easily in calm water. The nutritional quality of biofloc to cultured animals is good but rather variable. The dry-weight protein content of biofloc ranges from 25 to 50 percent, with most estimates between 30 and 45 percent. Fat content ranges from 0.5 to 15 percent, with most estimates between 1 and 5 percent. There are conflicting reports about the adequacy of bioflocs to provide the often limiting amino acids methionine and lysine. Bioflocs are good sources of vitamins and minerals, especially phosphorus. Bioflocs may also have probiotic effects. Dried bioflocs have been proposed as an ingredient to replace fishmeal or soybean meal in aquafeeds. The nutritional quality of dried bioflocs is good, and trials with shrimp fed diets containing up to 30 percent dried bioflocs show promise. Nonetheless, it is unlikely that dried bioflocs could replace animal or plant protein sources used in commercial-scale aquafeed manufacturing because only limited quantities are available. Furthermore, the costeffectiveness of producing and drying biofloc solids at a commercial scale is questionable.
What biofloc systems do
Bioflocs provide two critical services—treating wastes from feeding and providing nutrition from floc consumption. Biofloc systems can operate with low water exchange rates (0.5 to 1 percent per day). This long water residence time allows the development of a dense and active biofloc community to enhance the treatment of waste organic matter and nutrients. In biofloc systems, using water exchange to manage water quality is minimized and internal waste treatment processes are emphasized and encouraged. The advantages and disadvantages of biofloc systems compared to ponds and recirculating systems are summarized in Table 1. Research with shrimp indicates that culture water contains growth-enhancing factors, such as microbial and animal proteins, that boost production. Flocs are a supplemental food resource that can be grazed by shrimp or tilapia between feedings of pelleted diets. A potential benefit of biofloc systems is the capacity to recycle waste nutrients through microbial protein into fish or shrimp. About 20 to 30 percent of the nitrogen in added feed is assimilated by fish, implying that 70 to 80 percent of nitrogen added as feed is released to the culture environment as waste. In biofloc systems, some of this nitrogen is incorporated into bacterial cells that are a main component of biofloc. Consumption of this microbial protein, in effect for a second time, contributes to growth. Research with shrimp and tilapia suggests that for every unit of growth derived from feed, an additional 0.25 to 0.50 units of growth are derived from microbial protein in biofloc systems. In other words, 20 to 30 percent of shrimp or tilapia growth is derived from the consumption and digestion of microbial protein. This benefit is reflected in improved feed conversion, one of the best predictors of system profitability and business sustainability. However, the value of flocs in nutrition is limited at the highest levels of production intensity because the contribution of feed to growth of cultured animals is overwhelming.
Suitable culture species
A basic factor in designing a biofloc system is the species to be cultured. Biofloc systems work best with species that are able to derive some nutritional benefit from the direct consumption of floc. Biofloc systems are also most suitable for species that can tolerate high solids concentration in water and are generally tolerant of poor water quality. Species such as shrimp and tilapia have physiological adaptations that allow them to consume biofloc and digest microbial protein, thereby taking advantage of biofloc as a food resource. Nearly all biofloc systems are used to grow shrimp, tilapia, or carps. Channel catfish and hybrid striped bass are examples of fish that are not good candidates for biofloc systems because they do not tolerate water with very high solids concentrations and do not have adaptations to filter solids from water
Basic types of biofloc systems ————-
Few types of biofloc systems have been used in commercial aquaculture or evaluated in research. The two basic types are those that are exposed to natural light and those that are not. Biofloc systems exposed to natural light include outdoor, lined ponds or tanks for the culture of shrimp or tilapia and lined raceways for shrimp culture in greenhouses. A complex mixture of algal and bacterial processes control water quality in such “greenwater” biofloc systems. Most biofloc systems in commercial use are greenwater. However, some biofloc systems (raceways and tanks) have been installed in closed buildings with no exposure to natural light. These systems are operated as “brown- water” biofloc systems, where only bacterial processes control water quality. The specifications and performance of various biofloc production systems are discussed in more detail at the end of this publication.
Mixing and aeration———-
Intensive turbulent mixing is an essential requirement of biofloc systems. Solids must be suspended in the water column at all times or the system will not function. Without mixing, bioflocs settle out of suspension and may form piles that rapidly consume nearby dissolved oxygen. These anaerobic zones can lead to the release of hydrogen sulfide, methane, and ammonia that are highly toxic to shrimp and fish. Solids can be removed by periodic flushing or by pumping sludge from the pond center. Sludge banks are resuspended periodically by moving and repositioning paddlewheel aerators. Creating turbulent conditions in relatively small tanks or raceways is much easier than in larger outdoor ponds. Excessive turbulence can present a challenge to cultured animals by making it difficult for fish or shrimp to locate feed. Compared to water in aquaculture ponds or most recirculating systems, water in biofloc systems has an elevated respiration rate caused by a high concentration of suspended solids. In intensive, greenwater raceways for shrimp, water respiration rates range from 2 to 2.5 mg O2/L per hour, although it can be as high as 6 mg O2/L per hour. This does not include respiration by the fish or shrimp crop, which brings overall respiration to 5 to 8 mg O2/L per hour. Water respiration in indoor brownwater biofloc systems is normally about 6 mg O2/L per hour. It is absolutely essential to provide sufficient aeration or oxygenation to meet this high oxygen demand and to maintain oxygen concentration at safe levels. These high respiration rates also indicate that the response time in the event of a system failure is very short, often less than 1 hour. Thus, monitoring, alarms, and emergency power systems are required elements of biofloc systems. In practice, aeration is used to supply oxygen and provide mixing. Although paddlewheel aerators supply oxygen efficiently, they are not ideal for pond mixing. Devices that provide only mixing are rarely used. Various configurations of aeration equipment are possible, depending on the specific form of biofloc system. In lined ponds or tanks, multiple paddlewheel aerators are arrayed to provide whole-pond, circular mixing. Shrimp raceways in greenhouses often use banks of airlift pumps placed at intervals around raceways to aerate and circulate water. Diffused aeration can be used in small tanks. Devices that circulate water at low head, such as low-speed paddlewheels and airlift pumps, can be used. The power requirement for mixing and aeration far exceeds that for conventional ponds and most recirculating systems. Biofloc shrimp ponds are aerated with 25 to 35 hp/ha, and some intensive tilapia systems are aerated with 100 to 150 hp/ha. These intensive aeration rates could not be applied to earthen ponds without significant erosion; thus, most biofloc systems are lined. Biofloc systems are not a good choice in areas where power supplies are unreliable or electricity is expensive.
Effect of feeding rate and the greenwater-to-biofloc transition—————
A predictable sequence of changes occurs over time in sunlight-exposed biofloc systems as feeding rate is increased (Table 2; Fig. 2). At some point, a system will abruptly transition from a greenwater, algal system to a brownwater, bacterial system. The transition described here is based on conditions in an intensive greenhouse raceway for shrimp. Conditions leading to the transition from greenwater to brownwater biofloc will vary somewhat in different system types (i.e., ponds, raceways, tanks). As daily feeding rate increases from 100 to 200 kg/ ha (10 to 20 g/m2 ), the water will appear green with a dense algae bloom. Algal uptake is the main mechanism for ammonia control. The aerator power required at this feeding rate is about 25 to 30 hp/ha. At a daily feeding rate of 300 kg/ha, there is an abrupt shift when the lack of light at very high algal density hinders photosynthesis. Bacteria begin to grow and bioflocs develop, as indicated by an increase in suspended solids concentration (250 to 500 mg/L) and the associated rapid increase in water respiration (6 mg O2/L per hour). This requires a five-fold increase in aerator power from 30 to 150 hp/ha to match the oxygen demand. Most of this increased energy demand is required to maintain bioflocs in suspension. Despite these changes, the water continues to appear green and there is a slight surplus of oxygen produced. When the feeding rate is between 400 and 600 kg/ ha per day, the water appears green-brown. Beyond 700 kg/ha per day, the water appears brown with biofloc and there is essentially no contribution by algae. Further increases in feeding rate require correspondingly more aerator power. The transition is sometimes difficult to perceive visually. The functional shift from a surplus to a deficit of oxygen occurs while the water continues to look green. The color change from green to brown takes place after the transition from a mostly algal to a mostly bacterial biofloc system has occurred. So water color is not an accurate indicator of system status. At high aeration rates, the appearance of large amounts of surface foam is a good sign of a system in transition.
Ammonia dynamics
A major goal of water quality management in any aquatic animal production system is maintaining ammonia concentration below toxic levels. In biofloc systems, there are three main processes that control ammonia— algal uptake, bacterial assimilation, and nitrification. The transformations and dynamics of ammonia in biofloc systems are complex, involving interplay among the algae and bacteria that compete for ammonia. The relative importance of each process depends on many factors, among them the daily feeding rate, suspended solids (biofloc) concentration, ammonia concentration, light intensity, and input carbon-to-nitrogen (C:N) ratio.
Algal uptake
In any biofloc system exposed to sunlight, a dense algal bloom will develop in response to nutrient loading from feeding. Nutrients released from decomposing organic matter (including dead algae, fecal solids, and uneaten feed) are rapidly taken up and stored in algae cells. The rate of algal uptake in biofloc systems is mainly influenced by underwater light intensity. In biofloc systems with a primary dependence on algal uptake, extended periods of cloudy weather can cause spikes of ammonia concentration. The accumulation of biofloc solids shades out algae and limits ammonia uptake. Daily fluctuation in dissolved oxygen concentration and pH, despite intensive aeration, is another characteristic of biofloc systems where algal activity is predominant. Generally, at daily feeding rates less than 300 kg/ha (30 g/m2 ), algal activity is the major factor controlling water quality.
Bacterial assimilation
Many of the early names for biofloc systems included the word “heterotrophic,” which describes a group of bacteria that, by definition, obtains carbon from organic sources. Despite large inputs of feed to intensive systems, the growth of heterotrophic bacteria in biofloc systems is limited by dissolved organic carbon. To stimulate production of heterotrophic bacteria, the C:N ratio of inputs is raised by adding a supplemental source of carbohydrate or reducing feed protein level. By this manipulation, hetero trophic bacteria create a demand for nitrogen (as ammonia) because organic carbon and inorganic nitrogen are generally taken up in a fixed ratio that reflects the composition and requirement of bacterial cells. Thus, ammonia can be controlled by adding organic carbon to stimulate the growth of heterotrophic bacteria. Similar to algae, ammonia is “immobilized” while packaged in heterotrophic bacterial cells as protein. Because the growth rate of heterotrophic bacteria is so much greater than that of nitrifying bacteria, ammonia control through immobilization by heterotrophic bacteria occurs rapidly, usually within hours or days if a sufficient quantity of simple organic carbon (e.g., sugar or starch) is added. The packaging of nitrogen in bacterial cells is temporary because cells turn over rapidly and release nitrogen as ammonia when they decompose. Cells are also consumed by fish or removed as excess solids. As with nitrogen assimilated by algae, microbial protein in flocs containing heterotrophic bacteria can serve as a supplemental source of nutrition for fish and shrimp.
Nitrification
The two-step oxidation of ammonia to nitrate is called nitrification. The bacterial process transforms a toxic form of nitrogen (ammonia) to one that is toxic only at high concentrations (nitrate). Over time, nitrate accumulates in low-exchange biofloc systems. In contrast to rapid cycling between dissolved ammonia and algal or bacterial cells, nitrification is responsible for the long-term, ultimate fate of a large fraction (25 to 50 percent) of the nitrogen from feed added to intensive biofloc systems. This mechanism becomes relatively more important as production intensity, as measured by daily feeding rate, increases. To simplify the nitrogen dynamics in biofloc systems with low water exchange: Waste nitrogen is repeatedly cycled between dissolved ammonia and solids of algae or bacteria. If solids are removed, a significant fraction of added nitrogen can be taken out of the system. If solids are not removed, a large proportion of nitrogen (as ammonia) is ultimately oxidized to nitrate, which accumulates.
Management strategies for ammonia control in biofloc systems
Balancing input C:N ratio
In biofloc systems, a major factor that controls ammonia concentration is the C:N ratio of feed and other inputs. A feed with a 30 to 35 percent protein concentration has a relatively low C:N ratio, about 9 to 10:1. Increasing the C:N ratio of inputs to 12 to 15:1 favors the heterotrophic pathway for ammonia control. The low C:N ratio of feed can be augmented by adding supplemental materials with high C:N ratio. Or, the input C:N ratio can be increased by reducing feed protein content. Ammonia uptake by heterotrophic bacteria occurs rapidly after carbohydrate supplementation. Ammonia control through the heterotrophic pathway is often more stable and reliable than algal uptake or nitrification. Many practical and processed materials have been used as carbon sources in biofloc systems, including grain pellets, molasses, sugar cane bagasse, and chopped hay, among others. Carbohydrate materials should be low-cost and convenient. Organic matter that breaks down easily and quickly is best. Heterotrophic bacteria in biofloc systems can act on simple organic matter rapidly, within minutes to hours. Simple carbohydrates such as sugar (sucrose or dextrose) or starch will have the quickest effect. The best carbon source to add during system start-up, when the most rapid response is needed, is simple sugar. To promote exclusive control of ammonia concentration by the heterotrophic pathway, carbohydrate additions must be made in accordance with feeding rate. For every 1 kg of 30 to 38 percent protein feed added, add 0.5 to 1 kg of a carbohydrate source such as sugar. More carbohydrate is needed at the higher protein level. It is clear that relatively large quantities of carbohydrate must be added to control ammonia concentration this way. Less carbohydrate can be added if other ammonia removal pathways are operating simultaneously in a biofloc system. There are several drawbacks to continually adding organic carbon to control ammonia. This pathway encourages the production of bacterial solids, which accumulate. If not controlled, solids concentration may reach levels that cause gill clogging. More oxygen will be needed to support the respiratory demands of a greater bacterial load, and additional energy is needed to keep solids in suspension. High rates of water respiration (oxygen consumption) reduce response time in the event of system failure. Capacity must be added to remove, treat, and dispose of accumulated solids. Ongoing carbon supplementation is required to control ammonia with this approach. In order to stop carbon supplementation, a system must be “weaned.” Stopping the supplemental carbon abruptly before the nitrification pathway is sufficiently developed will lead to water quality instability and potentially detrimental spikes of ammonia and/or nitrite. Once carbon supplementation ceases, superintensive biofloc systems naturally tend toward the nitrification pathway of ammonia control.
Promoting suspended-growth nitrification
In contrast to the previous approach, encouraging suspended-growth nitrification requires no supplemental carbohydrate or consideration of input C:N ratio. This approach emphasizes nitrification over other pathways for ammonia control, using the nitrifying bacteria that are attached to suspended solids (and surfaces of the culture unit) to control ammonia. Well-mixed biofloc systems without carbohydrate supplementation tend to develop this mechanism of long-term ammonia control naturally. One of the main disadvantages of this approach is the consumption of alkalinity by nitrification. All three processes that control ammonia in biofloc systems consume alkalinity, but nitrification is responsible for most of those losses. Denitrification reactors can be used to recover some of the lost alkalinity, but they increase production cost. Regular liming is a requirement of biofloc systems managed with this approach.
System management during start-up
During start-up, changes in water quality in biofloc systems are remarkably similar to those in conventional recirculating systems. System start-up is characterized by time lags in peak concentrations of ammonia and then nitrite as the different populations of bacteria develop. If the feeding rate is increased too rapidly, concentrations of ammonia or (especially) nitrite can increase to the point where they become toxic and affect fish growth, feed conversion, disease resistance, or—in some cases—survival. The duration of start-up depends on a wide range of factors, including temperature, feeding rate scheduling, and pre-seeding of the system with the right kind and quantity of microbes. Acclimation protocols for biofloc systems have not been standardized, and many system operators have developed their own techniques through hard-won experience. Nitrifying bacteria can be grown in stand-alone tanks at high concentration and then added to rearing tanks before stocking. Adding sludge or water from a previously acclimated system is also an effective approach to “seeding” a new tank or pond, although the practice represents a biosecurity risk. Ammonia or nitrite peaks during start-up can be avoided or minimized by adding carbohydrate. To neutralize 1 mg/L of ammonia (as N), add 15 to 20 mg/L of sugar. Carbohydrate added during start-up to keep ammonia concentration low can extend the time required for system acclimation. Once the system is acclimated, further supplementation with carbon is optional because nitrifying bacteria are able to keep ammonia and nitrite concentrations at safe levels. Carbohydrate also can be added occasionally, as needed during the culture period, when ammonia concentration spikes.
Solids management
In biofloc systems, waste solids are allowed to accumulate and additional solids are encouraged by intensive aeration and carbohydrate additions. Over time, and with sufficient mixing, solids can accumulate to undesirably high levels (2,000 to 3,000 mg/L). Biofloc systems are typically operated at suspended solids concentrations less than 1,000 mg/L and most often less than 500 mg/L. A suspended solids concentration of 200 to 500 mg/L is sufficient for good system functionality and will control ammonia without excessive water respiration. The best feed consumption in shrimp raceway biofloc systems occurs at a solids concentration of 100 to 300 mg/L. Imhoff or settling cones are a simple way to index suspended solids concentration (Fig. 3). The cones have marked graduations on the outside that can be used to measure the volume of solids that settle from 1 liter of system water. The interval of time should be standardized and convenient, usually 10 to 20 minutes. Solids also can be measured with a turbidity meter. Maintaining a settleable solids concentration of 25 to 50 mL/L will provide good functionality in biofloc systems for tilapia. In lined biofloc shrimp ponds, 10 to 15 mL/L is the typical target range. Turbidity of 75 to 150 NTU is comparable to the recommended settleable solids concentration provided that color interference is not too severe. Solids concentration should be managed as a compromise between the functionality of the biofloc system as a biofilter (for ammonia control) and the oxygen demand of the water, which increases directly with solids concentration. In other words, the concentration should be as low as possible to provide sufficient biofiltration and not so high that the requirement for aeration and mixing power is excessive. Operating rearing tanks with relatively low suspended solids concentration reduces the risk of dissolved oxygen depletion associated with system failure by increasing response time. A relatively low suspended solids concentration also allows photosynthesis by algae to contribute to the oxygen supply.
Using settling tanks for solids control
Simple gravity settling tanks, also known as clarifiers, can be used to control solids concentration at high feeding rates in superintensive biofloc systems. Clarifiers can be operated intermittently whenever the assessment of solids concentration with Imhoff cones indicates that the target range has been exceeded. Alternatively, clarifiers can be operated continuously if sized so that a relatively small proportion of the tank volume is clarified each day. Good control of solids concentration can be achieved by operating clarifiers at a flow rate that turns over the rearing tank water every 3 to 4 days. In general, clarifier volume is 1 to 5 percent of system volume and is operated at a flow rate to provide a residence time of 20 to 30 minutes, which is sufficient to settle most heavy solids. Clarifiers are simple to use and effective at removing coarse, easily settled solids. However, the aggressive use of clarifiers to control suspended solids may leave fine solids or larger solids that do not settle readily in the system. Fine solids can be removed with foam fractionators or dissolved air flotation units. In practice, the size distribution of solids in biofloc systems is not managed. Management of biofloc solids is limited to controlling their retention time, although most biofloc systems have limited capacity to control solids concentration.
Liming for alkalinity management
Alkalinity is the capacity of water to buffer or resist changes in pH in response to additions of acid or base. Water in biofloc systems should be maintained with ample reserves of alkalinity because it is constantly depleted by reactions with acid added to water. The activity of nitrifying bacteria is responsible for most losses of alkalinity in intensive biofloc systems. Over time, acid produced by nitrification wears down the reserve of alkalinity in the water. Once alkalinity is depleted, pH can drop steeply, inhibiting bacterial function, including that of the important nitrifying bacteria. In that case, ammonia accumulates to the point where fish appetite and feeding response are curtailed. This limits daily feeding rate, feed conversion efficiency, and, ultimately, yield. Alkalinity should be kept between 100 and 150 mg/L as CaCO3 by regular additions of sodium bicarbonate. Other liming agents are less suitable. Caustic agents (e.g., calcium hydroxide) can be used with a continuous dosing system. In intensive, nitrification-dominated biofloc systems, every kilogram of feed added to the system should be supplemented with 0.25 kilogram of sodium bicarbonate. Even with regular additions, facility operators should have a regular (at least weekly) monitoring program to evaluate alkalinity.
Denitrification and sludge treatment
Alkalinity can be recovered in denitrification units. Nitrate accumulates in most intensive biofloc systems because of ongoing nitrification. If unchecked, nitrate concentration reflects the cumulative feed loading to the system. Nitrate accumulation can be tempered by dilution through water exchange, but this defeats the purpose of intensive water use and reduces biosecurity. Denitrification units are used as part of a water conservation and biosecurity strategy where it is also a cost issue to conserve salts. This is an acute need in superintensive saltwater systems for shrimp, especially those located inland. Furthermore, the discharge of saline effluent is restricted or regulated in many areas, especially inland. Denitrification units are operated under generally quiescent and anoxic conditions. Solids can be shunted to a side-stream tank and allowed to accumulate. A low flow of culture water, sufficient to provide a detention time of 1 to 2 days, is adequate to control nitrate concentration. Solids accumulation will reach a steady state. Under anoxic conditions, the steady supply of nitrate is used as an oxidant to continually oxidize organic matter, although simple organic carbon (sugar) may be needed to bolster the process. Bicarbonate is released by bacteria as a by-product of this process. Thus, the alkalinity that was lost from nitrification can be recovered by denitrification. Additional water can be conserved by using a sequencing batch reactor to reduce the volume of sludge and mass of solids discharged from an intensive aquaculture facility. The specific sequence of operational steps is: ■ Fill. A batch of sludge collected from settling tanks is added to the reactor. (Closed reactors work best but are not necessary; any tank or vessel is suitable.) ■ React. Solids and residual bioflocs are vigorously mixed and aerated for ½ to 1 day to promote solids degradation. ■ Settle. Mixing and aeration are stopped. Most solids will settle quickly and nearly all within 2 to 3 hours. ■ Decant. The clean water that overlies the settled solids is drawn off and returned to the biofloc system. This sequence is repeated for each additional batch of sludge. Water with a rotten-egg odor, indicating hydrogen sulfide, should not be returned to culture tanks before it is vigorously aerated. One variation of the process is to extend the settling period. Very quickly after settling, respiration by settled biofloc solids will fully consume all oxygen in the water. Anoxic conditions allow other reactions to occur, including denitrification. Operation is then alternated between an aerated, suspended, oxidized mode and a quiescent, settled, anaerobic mode. This alternation takes advantage of multiple bacterial pathways to break down organic matter.
Specifications and performance of biofloc systems
Lined ponds for commercial shrimp culture
Much of the interest in developing biofloc systems emerged from research at the Waddell Mariculture Center as applied to a commercial shrimp farm, Belize Aquaculture Limited, in the mid-1990s. Since then, the technique has been applied to ponds on large shrimp farms in Indonesia, Malaysia, and Australia. As mentioned previously, one major driving force for using biofloc technology in shrimp farming is concern about biosecurity, especially the control of white-spot and other viruses. The basic approach is to use relatively small (0.5- to 1.5-ha) ponds that are lined with plastic (usually 30- to 40-mil HDPE) and aerated intensively (28 to 32 hp/ha) with paddlewheel aerators to maintain floc in suspension. As a rule-of-thumb, one horsepower of paddlewheel aeration can support about 400 to 500 kg of shrimp. Aerator positioning is important and must be done to effect good circulation and avoid calm areas (quiescent zones) where sludge can accumulate. Aerators must be repositioned regularly to suspend settled solids and prevent toxic anaerobic zones. Biofloc concentration of 15 mL/L (as settleable solids) is maintained by adding grain pellets (18 percent protein) and molasses, resulting in an input C:N ratio greater than 15:1. When shrimp biomass reaches 10 metric tons/ ha, sludge should be drained from the center of ponds, if possible. Shrimp are stocked at high density (125 to 150 PL10 per m2 ). The maximum daily feeding rate before harvest is 400 to 600 kg/ha. After 90 to 120 days, yields of 20 to 25 metric tons/ha per crop of 18- to 20-g shrimp can be expected, although 15 to 20 metric tons/ha is probably more typical (Table 3). Almost 50 metric tons/ha have been produced in intensive shrimp biofloc ponds stocked at 280 per m2 . In comparison, conventional semi-intensive shrimp ponds can produce 4 to 8 metric tons/ha.
BIOFLOC TECHNOLOGY: A SUITABLE TOOL FOR BLUE REVOLUTION IN INDIA
Compiled & Shared by- Team, LITD (Livestock Institute of Training & Development)
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Reference-On Request.
